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KMID : 0369819930230030133
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Jorunal of Korean Pharmaceutical Sciences
1993 Volume.23 No. 3 p.133 ~ p.137
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High Performance Liquid Chromatographic Assay of Acebutolol and its Acetyl Metabolite in Plasma
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백채선/Baek CS
Emil T . Lin
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Abstract
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A high-performance liquid chromatographic assay using ion-pair reverse-phase system was developed for the separation of acebutolol and acebutolol acetyl metabolite in plasma. A ion-pair reverse-phase system consisting of an ODS-bonded silica column and a mixture of 20% CH₃CN, 0.1% H₃PO₄, 0.035 M heptanesulfonic acid and 0.005 M tetrabutylammonium hydrogen sulfate as the mobile phase were used. Triamterene was employed as an internal standard. Based on 0.2 ㎖ of plasma, the detection limits were 10.4 ng/㎖ for acebutolol and 10.3 ng/㎖ of acebutolol acetyl metabolite at the signal-to-noise ratio of 3:1.
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KEYWORD
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